TB-500 Dosing and Reconstitution — 2026 Ireland Research Reference

By the Peptides Lab Ireland research team · Updated July 2026 · For research use only

TB-500 is a synthetic 17 amino acid fragment of Thymosin Beta-4 with a large tissue-repair and cellular-migration research literature. This reference sets out reconstitution volumes, working concentrations and the technique details Irish research groups should follow for reproducible TB-500 protocols.

TB-500 fundamentals

• Class: Synthetic fragment of Thymosin Beta-4 (17 amino acids)
• Sequence: LKKTETQ (active site fragment)
• Format: Lyophilised powder
• Peptides Lab Ireland presentation: 2 mg per vial (also 5 mg + 10 mg in blend formats)
• Storage: Lyophilised −20 °C; reconstituted 2–8 °C
• Reconstitution solvent: Bacteriostatic water (0.9% benzyl alcohol)

Reconstitution volume reference : 2 mg TB-500 vial

Working concentration calculation

Standard formula: concentration (µg/ml) = (mg in vial × 1000) ÷ ml of diluent. Example: 2 mg TB-500 + 2 ml bacteriostatic water = 1000 µg/ml (1 mg/ml). A 500 µg protocol dose is 500 µl.

TB-500 research applications

Preclinical research literature on TB-500 covers:

• Cellular migration research : actin sequestration effects on cytoskeletal dynamics
• Wound healing models , dermal repair, muscle regeneration, tendon repair
• Cardiovascular repair research — endothelial cell repair, angiogenesis models
• Neuroregeneration research , neurite outgrowth, nerve repair models
• Muscle regeneration models — satellite cell activation, myofibre repair

Reconstitution technique

1. Warm both vials to room temperature.
2. Wipe stoppers with alcohol.
3. Draw the diluent volume with a 30G insulin syringe.
4. Add slowly against the vial wall.
5. Swirl gently. Don’t shake.
6. Allow to dissolve (typically < 60 seconds).
7. Label and refrigerate.

See our full reconstitution guide.

Stability and storage

Reconstituted TB-500 in bacteriostatic water is typically stable at 2–8 °C for 3–4 weeks. For extended protocols, aliquot single-use portions and freeze at −20 °C. See our cold chain guide.

TB-500 blends

TB-500 is a component of two pre-formulated blends stocked at Peptides Lab Ireland for tissue-repair research:

• Wolverine Stack — BPC-157 5mg + TB-500 5mg
• Glow Stack BPC-157 10mg + TB-500 10mg + GHK-Cu 50mg

See our blend comparison for research context.

Sourcing TB-500 in Ireland

Peptides Lab Ireland stocks HPLC-verified TB-500 with per-batch Certificate of Analysis independently issued by Optima Labs. See the product page. Fast Ireland delivery to Dublin, Cork, Galway, Limerick and nationwide.

Related research guides

• TB-500 Ireland . Research Reference
• BPC-157 vs TB-500 , with How the Two Compounds Differ
• Wolverine Stack vs Glow Stack
• How to Read a Peptide COA

All compounds supplied by Peptides Lab Ireland are for in-vitro laboratory research and educational use only. Not intended for human or veterinary use.