By the Peptides Lab Ireland research team · Updated July 2026 · Research use only
Semaglutide is the GLP-1 receptor mono-agonist reference compound in modern metabolic research literature. This reference sets out reconstitution volumes, working concentrations and Irish research protocol context.
Semaglutide fundamentals
- Class: GLP-1 receptor mono-agonist
- Structure: Modified GLP-1(7-37) analogue with γ-glutamyl-C18 di-acid at Lys26 for albumin binding
- Amino acids: 31
- Molecular weight: ≈ 4113.6 Da
- Peptides Lab Ireland presentation: 5 mg per vial
- Storage: Lyophilised −20 °C; reconstituted 2–8 °C
Reconstitution reference — 5 mg Semaglutide vial
| Bacteriostatic water | Working concentration | Volume for common research doses |
|---|---|---|
| 1.0 ml | 5 mg/ml (5000 µg/ml) | 250 µg → 50 µl · 500 µg → 100 µl · 1 mg → 200 µl |
| 2.0 ml | 2.5 mg/ml (2500 µg/ml) | 250 µg → 100 µl · 500 µg → 200 µl · 1 mg → 400 µl |
| 2.5 ml | 2 mg/ml (2000 µg/ml) | 250 µg → 125 µl · 500 µg → 250 µl |
| 5.0 ml | 1 mg/ml (1000 µg/ml) | 250 µg → 250 µl · 500 µg → 500 µl |
Research protocol context
Semaglutide research protocols operate across a wide dose range depending on the model. Cell-culture receptor pharmacology uses nM to sub-nM concentrations in assay buffer. Rodent metabolic models use weight-normalised doses in µg/kg to mg/kg ranges. Molar-equivalent dosing is used for comparative studies against Tirzepatide, Retatrutide and other agonist-class references.
Reconstituted stability
Semaglutide reconstituted in bacteriostatic water is stable at 2–8 °C for approximately 4 weeks. For longer studies, aliquot immediately after reconstitution and freeze at −20 °C. See our cold chain guide.
Regulatory context , Ireland
Semaglutide is a current HPRA enforcement focus in Ireland. All Semaglutide sold at Peptides Lab Ireland is supplied strictly for in-vitro laboratory research and educational use — see our HPRA guide.
Comparison with related GLP-1-class compounds
- Semaglutide vs Tirzepatide: Mono vs dual agonist — see our receptor-class comparison
- Semaglutide + Cagrilintide: co-formulated research compound (amylin analogue combined with GLP-1 agonism)
Sourcing Semaglutide in Ireland
HPLC-verified Semaglutide with per-batch Certificate of Analysis (Optima Labs). See the product page.
See also
- Semaglutide Ireland , Research Reference
- GLP-1, GIP and Glucagon Receptor Agonists Compared
- Reconstitution guide
For laboratory research use only. Not intended for human or veterinary use.