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Semaglutide Dosage Chart — Ireland Research Reference (2026)

By the Peptides Lab Ireland research team · Updated July 2026 · Research use only

Semaglutide is the GLP-1 receptor mono-agonist reference compound in modern metabolic research literature. This reference sets out reconstitution volumes, working concentrations and Irish research protocol context.

Semaglutide fundamentals

  • Class: GLP-1 receptor mono-agonist
  • Structure: Modified GLP-1(7-37) analogue with γ-glutamyl-C18 di-acid at Lys26 for albumin binding
  • Amino acids: 31
  • Molecular weight: ≈ 4113.6 Da
  • Peptides Lab Ireland presentation: 5 mg per vial
  • Storage: Lyophilised −20 °C; reconstituted 2–8 °C

Reconstitution reference — 5 mg Semaglutide vial

Bacteriostatic water Working concentration Volume for common research doses
1.0 ml 5 mg/ml (5000 µg/ml) 250 µg → 50 µl · 500 µg → 100 µl · 1 mg → 200 µl
2.0 ml 2.5 mg/ml (2500 µg/ml) 250 µg → 100 µl · 500 µg → 200 µl · 1 mg → 400 µl
2.5 ml 2 mg/ml (2000 µg/ml) 250 µg → 125 µl · 500 µg → 250 µl
5.0 ml 1 mg/ml (1000 µg/ml) 250 µg → 250 µl · 500 µg → 500 µl

Research protocol context

Semaglutide research protocols operate across a wide dose range depending on the model. Cell-culture receptor pharmacology uses nM to sub-nM concentrations in assay buffer. Rodent metabolic models use weight-normalised doses in µg/kg to mg/kg ranges. Molar-equivalent dosing is used for comparative studies against Tirzepatide, Retatrutide and other agonist-class references.

Reconstituted stability

Semaglutide reconstituted in bacteriostatic water is stable at 2–8 °C for approximately 4 weeks. For longer studies, aliquot immediately after reconstitution and freeze at −20 °C. See our cold chain guide.

Regulatory context , Ireland

Semaglutide is a current HPRA enforcement focus in Ireland. All Semaglutide sold at Peptides Lab Ireland is supplied strictly for in-vitro laboratory research and educational use — see our HPRA guide.

Comparison with related GLP-1-class compounds

  • Semaglutide vs Tirzepatide: Mono vs dual agonist — see our receptor-class comparison
  • Semaglutide + Cagrilintide: co-formulated research compound (amylin analogue combined with GLP-1 agonism)

Sourcing Semaglutide in Ireland

HPLC-verified Semaglutide with per-batch Certificate of Analysis (Optima Labs). See the product page.

See also

For laboratory research use only. Not intended for human or veterinary use.

Picture of Emma Louise

Emma Louise

Chief Compliance Officer at Peptides Lab Ireland. Emma Louise leads regulatory compliance, HPRA framework interpretation, batch quality documentation and editorial standards for the Peptides Lab Ireland research reference library. All research guides are reviewed under her editorial oversight.
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